Ultrastructure, Solubilization and Protein Composition of Eggshell (Chorion) of Gesonula punctifrons (Stal, 1861) (Orthoptera: Acrididae)

Authors

  • Arpita SHYAM ROY Tripura University
  • Durgadas GHOSH Tripura University

Keywords:

Gesonula punctifons, eggshell, SEM, TEM, SDS-PAGE.

Abstract

Developmental changes in ultrastructure and polypeptide composition of chorion of Gesonula punctifrons (Orthoptera: Acrididae) have been studied by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE). Along with morphogenesis and maturation of chorion pentagonal and hexagonal architecture and ‘cap’ like structure appeared in the surface of the eggshell. Transmission Electron Microscopic studies revealed presence of protein fiber deposition and their intercalation with polysaccharides. SDS-PAGE studies showed presence of five polypeptide bands in all the developmental stages and suggested possibilities of aggregate formation among different polypeptide components.

Author Biographies

Arpita SHYAM ROY, Tripura University

PhD Scholar, Department of Zoology, Tripura University

Durgadas GHOSH, Tripura University

Professor of Zoology, Department of Zoology, Tripura University

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Published

2014-12-03

How to Cite

SHYAM ROY, A., & GHOSH, D. (2014). Ultrastructure, Solubilization and Protein Composition of Eggshell (Chorion) of Gesonula punctifrons (Stal, 1861) (Orthoptera: Acrididae). Journal of the Entomological Research Society, 16(3), 45–54. Retrieved from https://www.entomol.org/journal/index.php/JERS/article/view/661

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Section

Journal of the Entomological Research Society