Effects of Parasitism and Envenomation by Pimpla turionellae (Hymenoptera: Ichneumonidae) on Hemolymph Free Amino Acids of Galleria mellonella (Lepidoptera: Pyralidae)

Authors

  • Olga Sak Balikesir University
  • Fevzi Uçkan Kocaeli University
  • Ekrem Ergin Gülhane Military Medical Academy
  • Hülya Altuntaş Anadolu University
  • Aylin Er Balikesir University

Keywords:

Wasp venom, parasitization, hemolymph, HPLC, amino acids

Abstract

The effects of dose-dependent envenomation by and parasitization of Pimpla turionellae Linnaeus (Hymenoptera: Ichneumonidae) on the ratio of hemolymph free amino acids of the host species Galleria mellonella Linnaeus (Lepidoptera: Pyralidae) pupae and larvae were investigated. Of the seventeen different free amino acids detected in the hemolymph of host pupae and larvae by high performance liquid chromatography, the ratio of free amino acids from parasitized and envenomated host pupae did not differ much when compared with those of unparasitized, null- or PBS-injected controls at different time points post-treatments. The exceptions to this trend were an increase in parasitized host pupae for glutamic acid with regard to other experimental groups at 4 and 8 h and a decrease in parasitized host pupae for leucine with regard to 0.01 and 0.05 VRE at 24 h post-treatments. In contrast to pupae, hemolypmh free amino acids of G. mellonella larvae differed upon venom injection among treatments and at different time points post-treatments. The ratios of alanine and leucine at 8 h and glutamic acid, serine, glycine+glutamine, valine, methionine, and phenylalanine at 24 h post-treatments differed from those of controls in treatment groups. However, there appeared no changes in the ratio of hemolypmh free amino acids in host larvae at 4 h post-treatments. Our study indicated that parasitism and experimental envenomation of G. mellonella by wasps resulted in different effects in the quantity of free amino acids depending on host developmental stage.

Author Biographies

Olga Sak, Balikesir University

Biology

Fevzi Uçkan, Kocaeli University

Biology

Ekrem Ergin, Gülhane Military Medical Academy

Nursing School

Hülya Altuntaş, Anadolu University

Biology

Aylin Er, Balikesir University

Biology

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Published

2014-03-31

How to Cite

Sak, O., Uçkan, F., Ergin, E., Altuntaş, H., & Er, A. (2014). Effects of Parasitism and Envenomation by Pimpla turionellae (Hymenoptera: Ichneumonidae) on Hemolymph Free Amino Acids of Galleria mellonella (Lepidoptera: Pyralidae). Journal of the Entomological Research Society, 20(1), 1–20. Retrieved from https://www.entomol.org/journal/index.php/JERS/article/view/510

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Section

Journal of the Entomological Research Society