Phylogenetic Analysis on Some Iranian White Grubs with New Data about Natural Pathogen of Polyphylla adspersa

Authors

  • Javad Karimi Department of Plant Protection , Faculty of Agriculture, Ferdowsi University of Mashhad
  • Reyhane Darsouei Ferdowsi university of Mashhad ,Iran
  • Somayeh Fattaho-hosseini Department of Plant protection,University of Tehran, Karaj
  • Hussein Sadeghi Department of plant protection, Faculty of Agriculture, Ferdowsi University of Mashhad

Keywords:

Polyphylla, Melolontha, Heterorhabditis, Natural Entomopathogen, DNA Barcode, Khorasan

Abstract

Three species of white grubs, Polyphylla olivieri, P. adspersa and Melolontha melolontha (Scarabaeidae: Melolonthinae) are among the serious economically important pests in Iran. Larval identification of those species is a difficult issue. Here, we provided DNA barcodes for these species of scarabids using mitochondrial DNA cytochrome oxidase I (COI). The relationship between these three species of Iranian white grubs and related species was addressed. Among these species, P. adspersa is the predominant species in North-East of Iran, Khorasan provinces.The isolation of an unknown pathogen indicated the incidence of a natural infection by a species of entomopathogenic nematode from Heterorhabditis genus. Classic data as well as molecular characterization of this insect pathogen using ITS sequences confirmed the occurrence of H. bacteriophora in natural populations of P. adspersa. This is the first data about DNA barcodes of Iranian white grubs as well as new record for natural entomopathogen of P. adspersa larvae.

Author Biographies

Javad Karimi, Department of Plant Protection , Faculty of Agriculture, Ferdowsi University of Mashhad

Plant Protection

Reyhane Darsouei, Ferdowsi university of Mashhad ,Iran

Plant Protection

Somayeh Fattaho-hosseini, Department of Plant protection,University of Tehran, Karaj

Plant Protection

Hussein Sadeghi, Department of plant protection, Faculty of Agriculture, Ferdowsi University of Mashhad

Plant Protection

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Published

2012-03-12

How to Cite

Karimi, J., Darsouei, R., Fattaho-hosseini, S., & Sadeghi, H. (2012). Phylogenetic Analysis on Some Iranian White Grubs with New Data about Natural Pathogen of Polyphylla adspersa. Journal of the Entomological Research Society, 14(1), 1–13. Retrieved from https://www.entomol.org/journal/index.php/JERS/article/view/286

Issue

Section

Journal of the Entomological Research Society