Expression of the Hsp40, Hsp70 and Hsp90 proteins in Colorado Potato Beetle (Leptinotarsa decemlineata Say) After the Dimethoate Treatment

Abstract

 Hsp40, Hsp70 and Hsp90 proteins exhibit increased level of expression in animals exposed to negative environmental conditions, including pesticides, but the measured effect depends on the class which particular Hsp is belonging to. In this study we examined Hsp level in the Colorado potato beetle exposed to pesticide dimethoate by using Western blotting and ELISA method. The highest level of expression was exhibited by Hsp90, while Hsp70 showed the lowest level of expression. There were no statistically significant differences in the expressions of Hsp40 and Hsp70 after the pesticide treatment. The highest concentration of Hsp90 was found in beetles 24 hours after the exposition to dimethoate, while the lowest was observed in insects 1 hour after the treatment. Hsps play a role in response to organophosphorous pesticide in Leptinotarsa decemlineata (Say, 1824), but there is not any simple correlation between Hsp levels and time of exposition. Hsp90 seems to be the most suitable protein to examine pesticide-provoked stress.