Journal of the Entomological Research Society, Vol 19, No 3 (2017)

Influence of Red Kidney Bean Seed Proteins on Development, Digestive α-amylase Activity and Gut Protein Pattern of Leptinotarsa decemlineata (Say)

Shabnam Ashouri, Reza Farshbaf Pourabad, Figen Zihnioğlu, Ebru Kocadağ Kocazorbaz

Abstract


 The effects of red kidney bean, Phaseolus vulgaris cv. Goli (L.), seed proteinaceous extract on larval development, digestive α-amylase and gut protein pattern of Colorado potato beetle, Leptinotarsa decemlineata (Say) were investigated. The results showed that four proteinaceous ammonium sulfate precipitated fractions (30, 50, 70, and 100%) of bean seed extract caused significant inhibition, in vitro, on α-amylase activity of all larval stages and adults up to 69%. Affinity chromatography using larval gut sample as ligand and silicon dioxide as matrix, were used to identify enzyme inhibitor proteins, but purified protein was not recognized on SDS-PAGE. Alpha-amylase activity of larvae fed on the treated leaves of four different potato cultivars (Agria, Burren, Picasso, and Marx) by bean seed extract was inhibited significantly up to the 60% on Burren. In the bioassay, weight of fourth instar larvae was reduced 19 mg and the larval growth was decreased by 45.35% on Picasso, as compared to the control. In two-dimensional electrophoresis and SDS-PAGE, the gut protein patterns were changed in effects of feeding on leaves treated with bean seed extract. These data revealed that red kidney bean seed extract suppressed digestive α-amylase activity in vitro and in vivo, caused side effects on the actual parameters monitored and had an impact on the gut protein pattern of L. decemlineata.


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