Genetic Variability of the Giant Black Aphid, Pterochloroides persicae (Hemiptera: Aphididae), Based on Sequences of the Mitochondrial Cytochrome b Gene

  • Imen KHARRAT Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (IR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar.
  • Maha MEZGHANI-KHEMAKHEM Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (IR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar
  • Dhia BOUKTILA Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (IR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, Tunisia / Institut Supérieur de Biotechnologie de Béja (ISBB), Université de Jendoub
  • Hanem MAKNI Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (IR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, Tunisia / Institut Supérieur de l’Animation pour la Jeunesse et la Culture de Bir-El
  • Mohamed MAKNI Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (IR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, Tunisia
Keywords: Aphididae, Pterochloroides persicae, phylogeny, mtDNA, invasive pest

Abstract

The giant black aphid Pterochloroides persicae (Cholodkovsky) is one of the most damaging pests of Prunaceous trees. It has been reported in North Africa since 1987. In this study, we have used sequences of the mitochondrial cytochrome b gene (Cytb), to elaborate the first phylogenetic analysis of this polyphagous pest. Sequences were obtained from 34 Tunisian clones covering different locations and different host plants. A total of 25 haplotypes were identified, among which 21 were unique. Haplotype H7 that was the most frequent would be the ancestral one. The maximum likelihood phylogenetic tree revealed that clones originating from distinct locations clustered into a single consistent group with little variations, reflecting the loss of genetic diversity that would have followed introduction of this pest. These results will have important implications for future studies of the genetic structure of this pest and other closely-related species.

Published
2014-08-04
How to Cite
KHARRAT, I., MEZGHANI-KHEMAKHEM, M., BOUKTILA, D., MAKNI, H., & MAKNI, M. (2014). Genetic Variability of the Giant Black Aphid, Pterochloroides persicae (Hemiptera: Aphididae), Based on Sequences of the Mitochondrial Cytochrome b Gene. Journal of the Entomological Research Society, 16(2), 99-109. Retrieved from http://www.entomol.org/journal/index.php/JERS/article/view/671
Section
Journal of the Entomological Research Society