Synergistic Activity of Andrographis paniculata Nees with Bacilus thuringiensis var israelensis Against Malarial Vector, Anopheles stephensi Liston (Diptera: Culicidae)

  • Kuppusamy Chennippan Bharathiar university
  • Vasugi Chellamuthu Bharathiar University
  • Abirami Dhandapani Bharathiar University
  • Murugan Kadarkarai Bharathiar University
Keywords: Andrographis paniculata, Anopheles stephensi, Bacillus thuringiensis, larvicidal activity, synergistic activity.

Abstract

Integrated vector control is an effective and essential part of any successful vector control program. The synergistic activity between the current effective pesticides and phytochemicals is one such strategy and powerful tool for developing vector control strategies. Bacillus thuringiensis var israelensis (Bti) and ethanolic extracts (Ee) of Andrographis paniculata were both observed for their larvicidal as well as pupicidal activity against Anopheles stephensi Liston. Ee extract with lethal concentrations (LC50 and LC90) values of 36.56 and 53.04 p. p. m. after 24 h and LC50 22.28 and LC90 51.26 p. p. m. after 48h for fourth instar larvae, respectively. LC50 and LC90 for Bti were 0.0967 and 0.1003 p.p.m after 24h and 0.2944 and 0.4158 p. p. m. after 48 h of exposure for fourth instar larvae, respectively. Combined formulations were evaluated for synergistic activity and a 1:4 ratio of Bti and Ee was observed to be more effective than 1:2 and 1:1 ratios. However the larvicicdal activity is minimal when the mixed formulations contained an equal amount of both constituents (i. e a 1: 1 ratio). A ratio of 1: 4 of Bti and Ee of A. paniculata was 51.6 fold more toxic at the LC90 for larvicidal activity, than Bti alone and this high level of activity resulted from synergism between the Bti and Ee of A. paniculata Nees.

Published
2011-12-05
How to Cite
Chennippan, K., Chellamuthu, V., Dhandapani, A., & Kadarkarai, M. (2011). Synergistic Activity of Andrographis paniculata Nees with Bacilus thuringiensis var israelensis Against Malarial Vector, Anopheles stephensi Liston (Diptera: Culicidae). Journal of the Entomological Research Society, 13(3), 71-86. Retrieved from http://www.entomol.org/journal/index.php/JERS/article/view/335
Section
Journal of the Entomological Research Society